ISG15 antibody (AA 2-157) (HRP)

Catalog No. ABIN1046251

This Rabbit Polyclonal antibody specifically detects ISG15 in ELISA. It exhibits reactivity toward Human.

Quick Overview for ISG15 antibody (AA 2-157) (HRP) (ABIN1046251)

Target: ISG15 (ISG15 Ubiquitin-Like Modifier (ISG15))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ISG15 antibody is conjugated to HRP

Application: ELISA

Product Details anti-ISG15 Antibody (HRP)

Binding Specificity: AA 2-157

Purpose: ISG15 Antibody, HRP conjugated

Purification: Protein G purified

Purity: >95 %

Immunogen: Recombinant Human Ubiquitin-like protein ISG15 protein (2-157AA)

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid freeze and thaw cycles.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for ISG15

Target: ISG15 (ISG15 Ubiquitin-Like Modifier (ISG15))

Alternative Name: ISG15

Background:

Background: Ubiquitin-like protein that is conjugated to intracellular target proteins after IFN-alpha or IFN-beta stimulation. Its enzymatic pathway is partially distinct from that of ubiquitin, differing in substrate specificity and interaction with ligating enzymes. ISG15 conjugation pathway uses a dedicated E1 enzyme, but seems to converge with the Ub conjugation pathway at the level of a specific E2 enzyme. Targets include STAT1, SERPINA3G/SPI2A, JAK1, MAPK3/ERK1, PLCG1, EIF2AK2/PKR, MX1/MxA, and RIG-1. Deconjugated by USP18/UBP43. Shows specific chemotactic activity towards neutrophils and activates them to induce release of eosinophil chemotactic factors. May serve as a trans-acting binding factor directing the association of ligated target proteins to intermediate filaments. May also be involved in autocrine, paracrine and endocrine mechanisms, as in cell-to-cell signaling, possibly partly by inducing IFN-gamma secretion by monocytes and macrophages. Seems to display antiviral activity during viral infections. Ref.12 Ref.13 Ref.14 Ref.15 Ref.20 Ref.21In response to IFN-tau secreted by the conceptus, may ligate to and regulate proteins involved in the release of prostaglandin F2-alpha (PGF), and thus prevent lysis of the corpus luteum and maintain the pregnancy

Aliases: G1P2 antibody, hUCRP antibody, IFI 15 antibody, IFI15 antibody, Interferon alpha inducible protein antibody, Interferon induced protein 15 antibody, Interferon induced protein IFI-15K antibody, Interferon stimulated protein 15 kDa antibody, Interferon, alpha-inducible protein (clone IFI-15K) antibody, Interferon-induced 15 kDa protein antibody, Interferon-induced 17 kDa protein antibody, Interferon-induced 17- kDa/15- kDa protein antibody, IP17 antibody, ISG 15 antibody, ISG15 antibody, ISG15 ubiquitin like modifier antibody, ISG15_HUMAN antibody, Ubiquitin cross-reactive protein antibody, Ubiquitin-like protein ISG15 antibody, UCRP antibody

UniProt: P05161